Q. 21 4.3( 6 Votes )

When the gene product is required in large amounts, so transformed bacteria with the plasmid inside the bacteria are cultured on a large scale in an industrial fermenter which then synthesizes its protein. This product is extracted from the fermenter for commercial use.

a) Why is the used medium drained out from one side while fresh medium is added from the other?

(b) List any four optimum conditions for achieving the desired product in a bioreactor.

Answer :

(a) This is to maintain the cells in the medium in their log/exponential phase. This is physiologically the most active phase.

(b) Temperature, pH, substrate and oxygen are the four optimum conditions required for achieving the desired product in a bioreactor.


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