The most important and the only physical barrier of a cell in the protoplast fusion experiment is the cell wall.
This physical barrier is overcome by its breakdown. The breakdown of the cell wall is done by digesting the cell wall. The digestion of the cell wall is done by certain enzymes known as cell wall digesting enzymes. Certain cell wall digesting enzymes are cellulose, hemicellulase, pectinase etc.
After the digestion of the cell wall the protoplast becomes naked and now the fusion of two naked protoplasts takes place to form a hybrid.
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