To isolate protoplast, one needs:
C. both pectinase and cellulase
The cell wall of plants is chemically made of cellulose and pectin. In order to digest the cell wall to isolate protoplast, the cell wall digesting enzymes are used. Pectinase breaks down pectin and cellulose breaks down cellulose.
Hence, (c) is the correct option.
Rate this question :
Discuss how the property of plant cell totipotency has been utilised for plant propagation and improvementBiology - Exemplar
What are the advantages of tissue culture methods over conventional method of plant breeding in crop improvement programmes?Biology - Exemplar
What are the physical barriers of a cell in the protoplast fusion experiment? How are the barriers overcome?Biology - Exemplar
Plants raised through tissue cultures are clones of the ‘parent’ plant. Discuss the utility of these plants.Biology - Exemplar
“Give me a living cell of any plant and I will give you a thousand plants of the same type” Is this only a slogan or is it scientifically possible? Write your comments and justify them.Biology - Exemplar