• The method used for the determination of the genotype of an organism is called test cross.
• In this type of cross, organism with unknown genotype is crossed with the recessive trait parents.
• If the all the progenies obtained are of same type then it is said that the organism with unknown genotype is homozygous.
• It is concluded that the genotype of unknown organism is heterozygous, if 50-50% result is obtained.
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