Q. 8

The most importan A. Origin of replication (ori)

B. Presence of a selectable marker

C. Presence of sites for restriction endonuclease

D. Its size

Answer :

Ori is the most important site in a vector due to following reasons:

a) It controls the copy number.

b) It’s the site for the insertion of gene of interest.

c) Ori site initiates replication of DNA.

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