Q. 105.0( 2 Votes )

Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Answer :

The 5 tools for rDNA technology are as follows


a) Restriction endonuclease - It cuts the DNA sequence at specific positions.


b) Vector - It is the most important tool for the rDNA technology vector contains site for the insertion of gene of interest called the origin of replication.


c) DNA ligase - It facilitates the joining of desired DNA with vector DNA.


d) Polymerase - The enzyme makes multiple copies of rDNA.


e) Host cell - The cell which takes up rDNA & change itself to transformant.


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