Q. 17

Name and describe

Answer :

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by the technique named gel electrophoresis.

Description of Gel electrophoresis

• DNA fragments are negatively charged molecules so they can be separated by forcing them to move towards the anode under electric field through a medium/matrix.

• Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds.

• The DNA fragments separate according to their size through sieving effect provided by the matrix.

• Hence, the smaller the fragment size, the farther it moves.

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