Q. 244.4( 24 Votes )

List the steps of preparation of temporary mount of a leaf peel to observe stomata.

Answer :


1. A healthy leaf is removed from a potted plant.


2. The peel from the lower surface of the leaf was removed with the help of forceps and kept in a watch glass containing water.


3. A few drops of safranin stain were put in the watch glass.


4. After a few minutes the peel was taken out and placed onto a clean glass slide.


5. Next, a drop of glycerine was put over the peel and a clean coverslip was gently placed over it with the help of a needle.


6. Excess stain and glycerin were removed with the help of a blotting paper.


7. The slide was observed under a microscope.


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