Answer :

In a dihybrid cross, if you get 9:3:3:1 ratio it denotes that the alleles of two genes are segregating independently. This ratio is obtained when both the parents are heterozygous for both the genes. According to Mendel’s law of Independent Assortment ‘when two pairs of traits are combined in a hybrid segregation of one pair of characters is independent of the other pair of characters.’

The ratio 9:3:3:1 represent all the possible four phenotypes, which in turn proves that during gamete formation, allels of the two different genes are separated independent of each other. And thus each phenotype results in a unique genotype.

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