Q. 115.0( 1 Vote )
Identify and expl
A- Denaturation, B- Annealing, C- extension
Denaturation- in this step double stranded DNA is denatured at high temperature of 940C for 15 seconds.
DNA strands separates, and act as template.
Annealing-Primers are added to anneal. This is done at 450 C using Mg2+ ions.
Extension- Taq polymerase is used to extend the primer by adding nucleotides at a temperature of 720C.
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