Q. 23

<span lang="EN-US

Answer :

Polymerase chain reaction is a method widely used in molecular biology to make many copies of a specific DNA segment. The steps involved are:

a. Denaturation: The double-stranded DNA is denatured by applying high temperature of 95°C for 15 seconds. Each separated single strand now acts as template for DNA synthesis.


b. Annealing: Two sets of primers are added which anneal to the 3′ end of each separated strand. Primers act as initiators of replication.


c. Extension: DNA polymerase extends the primers by adding nucleotides complementary to the template provided in the reaction. Taq polymerase, thermostable DNA polymerase, is used in the reaction.


All these steps are repeated many times to obtain several copies of desired DNA.




Figure 2. Steps involved in PCR.


Rate this question :

How useful is this solution?
We strive to provide quality solutions. Please rate us to serve you better.
Try our Mini CourseMaster Important Topics in 7 DaysLearn from IITians, NITians, Doctors & Academic Experts
Dedicated counsellor for each student
24X7 Doubt Resolution
Daily Report Card
Detailed Performance Evaluation
caricature
view all courses
RELATED QUESTIONS :

Explain three steBiology - Board Papers

<span lang="EN-USBiology - Board Papers

<span lang="EN-USBiology - Board Papers