A continuous culture system is maintained in a bioreactor by continuously and regularly feeding with culture medium steadily and by providing optimum growth conditions like pH, temperature, vitamins, salts, oxygen. It is also necessary to drain out the used medium from the other side.
In short, the used medium is drained out from one side of the bioreactor and the fresh medium is added from the other side
Continuous culture system is maintained in bioreactors to maintain the cells in their physiologically most active log/exponential phase.This type of culturing method produces a larger biomass leading to higher yields of desired protein.
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