Steps involved in insulin production using rDNA technology:
a. The gene which are known to synthesise A and B poly peptide chains are isolated.
b. It is inserted in the plasmid of the E. Coli bacteria, at a site where gene for beta- glactosidase synthesis is present, using a DNA ligase enzyme.
c. Now as the plasmid synthesise beta galactosidase, it produces A & B polypeptide chains.
d. These A&B polypeptide chains are isolated and combined by disulphide bonds.
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