In 1983, American company Eli Lily prepared human insulin by using the knowledge of r-DNA technology in the following steps-
a) DNA corresponding to A and B chain is prepared separately.
b) Plasmid bacteria are extracted.
c) Inserting DNA corresponding to A and B chain separately in plasmid.
d) Transforming the bacteria with this recombinant plasmid.
e) Desired product i.e. insulin is expressed from this DNA.
f) A and B chains are purified
g) They are linked together with disulphide bonds which makes them act like human insulin.
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