Q. 20

How did an Americ

Answer :


In 1983, American company Eli Lily prepared human insulin by using the knowledge of r-DNA technology in the following steps-


a) DNA corresponding to A and B chain is prepared separately.


b) Plasmid bacteria are extracted.


c) Inserting DNA corresponding to A and B chain separately in plasmid.


d) Transforming the bacteria with this recombinant plasmid.


e) Desired product i.e. insulin is expressed from this DNA.


f) A and B chains are purified


g) They are linked together with disulphide bonds which makes them act like human insulin.


Rate this question :

How useful is this solution?
We strive to provide quality solutions. Please rate us to serve you better.
Try our Mini CourseMaster Important Topics in 7 DaysLearn from IITians, NITians, Doctors & Academic Experts
Dedicated counsellor for each student
24X7 Doubt Resolution
Daily Report Card
Detailed Performance Evaluation
caricature
view all courses
RELATED QUESTIONS :

Explain how Eli LBiology - Board Papers

Mention the chemiBiology - Board Papers