Q. 20

How did an Americ

Answer :

In 1983, American company Eli Lily prepared human insulin by using the knowledge of r-DNA technology in the following steps-

a) DNA corresponding to A and B chain is prepared separately.

b) Plasmid bacteria are extracted.

c) Inserting DNA corresponding to A and B chain separately in plasmid.

d) Transforming the bacteria with this recombinant plasmid.

e) Desired product i.e. insulin is expressed from this DNA.

f) A and B chains are purified

g) They are linked together with disulphide bonds which makes them act like human insulin.

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