Q. 18

How are 'sticky e

Answer :

Sticky ends are produced by the enzyme Restriction endonuclease.

• Restriction endonuclease produce staggered cuts in which the two strands of a DNA double helix ae cleaved at a different locations this generates a protruding (3’ or 5’) ends, that is one strand of the double helix extends some bases beyond the other.

• Due to palindromic nature of the target sites the two ends produce such kind of sticky ends or cohesive ends.

• These are callrd sticky ends because they form hydrogen bonds with their complementary cut counterparts.

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