To create recombinant vectors -
The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
Since a restriction enzyme recognises and cuts the DNA at a particular sequence is called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source.
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