Answer :

The correct sequential order in which the steps should be followed in the artificial hybridisation programme are:

(a) Selection of parents is done first.

(b) Emasculation – after parents are selected, in bisexual female flower anthers are removed from flowed bud before the anther dehisces, this is called Emasculation. In case of female parent producing unisexual female flowers Emasculation step is not needed.

(c) Bagging – Now the stigma is bagged with butter paper bag to prevent its contamination with unwanted pollen. This is called Bagging.

(d) Collection of pollen from male parent – Now mature pollens are collected from other male parent is done.

(e) Dusting the pollen on stigma – When the stigma of bagged flower attains receptivity, the collected mature pollen grains are dusted on the stigma.

(f) Re-bagging – Now again the flower is re-bagged till the fruits are developed.

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