Answer :

The enzyme β-galactosidase helps in the selection of recombinants.

It identifies the recombinants from non recombinants by a process called insertional inactivation.

When a recombinant DNA is inserted in the coding sequence of enzyme β-galactosidase, the enzyme becomes inactive. This is known as insertional inactivation.

If the plasmid of the bacteria does not have an insert, then the blue color colonies are produced.

The production of blue colored colonies is due to chromogenic substances.

If the insert is present in the plasmid, then the enzyme becomes inactive and the colonies produced do not have any color.

In this way recombinant colonies are separated from non recombinants.

Also, selection of recombinants by inactivation of antibiotics is a very hectic procedure.

Hence, β-galactosidase enzyme site is a preferred selectable marker in comparison to antibiotic resistant selectable marker in biotechnology experiments.

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