Q. 25

Following a sever

Answer :

• DNA fingerprinting technique would help.

• It includes following steps:

1. Isolation of DNA

2. digestion of DNA by restriction endonucleases,

3. separation of DNA fragments by electrophoresis,

4. transferring(blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon,

5. hybridization using labeled VNTR probe and

6. detection of hybridized DNA fragments by autoradiography.


Figure.Schematic represtation of DNA fingerprinting.

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