The role of heat, primers and the bacterium Thermus aquaticus in the process of PCR:
Heat: It denatures PCR. The ds DNA is heated at the temperature 95oC, both the strands separate.
Primers: Primers are the small oligonucleotides of varying length of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of new chain.
Thermus aquaticus: A specialized thermostable enzyme Taq DNA polymerase is isolated from the bacterium Thermus aquaticus which can tolerate high temperatures and forms new strand.
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