Answer :

A palindromic sequence is a sequence of double stranded DNA when read from either side of the double strand it is read the same. For example, “MADAM” that is, when we read it from either side, from forward as well as backward it is read the same. The same happens in case of a DNA double helix only difference is that it is read on two strands and when read from 3’ or 5’ on both strands it reads the same. The five palindromic sequences are:

1. 3’....CGTATACG....5’


5’....GCATATGC....3’


2. 3’....AAGCTT....5’ (restriction site of endonuclease Hind III)


5’....TTCGAA....3’


3. 3’....GAATTC....5’ (restriction site of endonuclease EcoRI)


5’....CTTAAG....3’


4. 3’....AGCT....5’ (restriction site of endonuclease Alu1)


5’....TCGA....3’


5. 3’....TTATTCGAATAA....5’


5’....AATAAGCTTATT....3’


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