Q. 18 C4.8( 4 Votes )

Give a brief description of the principles of the following techniques taking an example in each case.

Chromatography

Answer :

1. It is one of the most useful methods for the separation and purification of organic compounds.


2. Principle:- It is based on the difference in movement of individual components of a mixture through the stationary phase under the influence of mobile phase.


3. For example- A mixture of red and blue ink can be separated by chromatography. A drop of the mixture is placed on the chromatogram. The component of the ink, which is less adsorbed on the chromatogram moves with the mobile phase while the less adsorbed component remains almost stationary.


4. The diagram is shown below:



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