Answer :

MATERIALS REQUIRED


Leaf of tradescantia or periwinkle or balsam, slides, coverslips, forceps, needles, cotton cloth, brush, blotting paper, watch glass, blade, dropper, glycerine, safranin


THEORY


1. Stomata (singular: stoma) are tiny pores found in the epidermis of leaves of the green plant through which exchange takes place.


2. Each stoma is bounded by two guard cells which unlike the other epidermal cells, possess chloroplast.


3. The walls of guard cells are thicker and more elastic, i.e., they are thickened on the inner side and thinner and more elastic on the outer side.


4. The guard cells control the opening and closing of stomata.


5.
When water flows into the guard cell, they swell expand and cause the stomatal pore to open. On the contrary, when they lose water, they shrink and close the stomatal pore.


PROCEDURE


1. Take a leaf of the green plant and remove the peel from its lower surface by tearing the leaf.


2. Place the leaf peel in the watch glass containing water, so that the peel does not dry during the experiment.


3. Add 1-2 drops of safranin to the watch glass containing the peel of leaf to stain.


4. Select a thin leaf peel and place it in the centre of a clean slide with the help of the brush


5. Put few drops of glycerine on the slide over the peel with help of a dropper.


6. Now, using a needle gently place a coverslip over the strained peel slide.


7. Remove the excess stain and glycerine with a blotting paper.


8. first, observe the slide under the lower magnification (i.e., 10 X) of a compound microscope and then, under the higher magnification (i.e., 45 X).


OBSERVATIONS


1. Epidermis or leaf peel consists of many cells which are irregular in outline and are arranged in a single layer with no intercellular spaces.


2.  These tiny pores are called as stomata and are seen in the epidermal cells.


3. Each stoma consists of two kidney-shaped guard cells.


4. Each guard cell has a nucleus and chloroplasts.


5. The diagram of the leaf peel shown below:




RESULT



Minute apertures called stomata are well observed in the temporary mount of leaf peel.


PRECAUTIONS


1. The peel should be freshly plucked from the leaf.


2. The peel should not be allowed to dry.


3. Leaf peel should not be over stained.


4. The slide to be used must be clean.


5. The peel should be placed in the centre of the slide for best view.

Rate this question :

How useful is this solution?
We strive to provide quality solutions. Please rate us to serve you better.
Related Videos
Magnetic Field of Circular ConductorMagnetic Field of Circular ConductorMagnetic Field of Circular Conductor41 mins
Electric MotorElectric MotorElectric Motor37 mins
Magnetic Effects of Electric CurrentMagnetic Effects of Electric CurrentMagnetic Effects of Electric Current44 mins
Lifelines of National Economy + Manufacturing Industries RevisionLifelines of National Economy + Manufacturing Industries RevisionLifelines of National Economy + Manufacturing Industries Revision47 mins
Electromagnetic  InductionElectromagnetic  InductionElectromagnetic Induction38 mins
Magnetic ForceMagnetic ForceMagnetic Force48 mins
Indicators show the Nature of Acids & Bases. How?Indicators show the Nature of Acids & Bases. How?Indicators show the Nature of Acids & Bases. How?34 mins
Heating Effect of Electric CurrentHeating Effect of Electric CurrentHeating Effect of Electric Current42 mins
Devour Parts of SpeechDevour Parts of SpeechDevour Parts of Speech45 mins
Pair of Linear Equations-Yahaan se laye marksPair of Linear Equations-Yahaan se laye marksPair of Linear Equations-Yahaan se laye marks57 mins
Try our Mini CourseMaster Important Topics in 7 DaysLearn from IITians, NITians, Doctors & Academic Experts
Dedicated counsellor for each student
24X7 Doubt Resolution
Daily Report Card
Detailed Performance Evaluation
caricature
view all courses
RELATED QUESTIONS :

Name the plant hoScience Lab Manual

Give an Example oScience Lab Manual

A, Band CarLakhmir Singh & Manjit Kaur- Biology

Give two major diScience Lab Manual