A. Two methodologies of HGP are:
i. Expressed Sequence Tags (EST's): In this method all the genes are identified which are expressed as RNA.
ii. Sequence annotation: In this method the whole set of genomes that contains all the coding and non-coding sequences are sequenced and later assigning different regions in the sequence with functions.
B. 'YAC' stands for Yeast Artificial Chromosome. It is used as a cloning vector for cloning DNA fragments in suitable host.
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