Q. 175.0( 4 Votes )

a) How do DNA fra

Answer :

(a) Separation of DNA fragments is done by gel electrophoresis. The DNA fragments are negatively charged and hence are separated by forcing them to move towards a positive end (anode) under an electric field through a medium. This medium or matrix is a natural polymer extracted from sea weeds: agarose.

The agarose gel provides a sieving effect by which DNA fragments are separated according to their size. The smaller fragments of DNA thus move to a farther distance.

(b) Lane 1 shows the migration of undigested DNA fragments. They are a mixture of DNA fragments produced by the random cutting of the DNA and hence appear as different sized fragments.

Lane 2, 3, 4 shows the digested set of DNA fragments, where the restriction endonucleases cut the DNA at specific sites along the DNA chain and fragments thus obtained are same sized.

(c) Staining the DNA with ethidium bromide and then exposing them to UV radiation can make pure DNA fragments observable in visible light.

Rate this question :

How useful is this solution?
We strive to provide quality solutions. Please rate us to serve you better.
Try our Mini CourseMaster Important Topics in 7 DaysLearn from IITians, NITians, Doctors & Academic Experts
Dedicated counsellor for each student
24X7 Doubt Resolution
Daily Report Card
Detailed Performance Evaluation
view all courses

Explain three steBiology - Board Papers

<span lang="EN-USBiology - Board Papers

<span lang="EN-USBiology - Board Papers