Q. 24

(a) Explain the p

Answer :

a) The fragmented DNA is place in wells in an agarose gel. Since DNA being negatively charged in nature movies towards the positive end of the plate in Electrophoresis machine. And while moving along the gel it sieves and gets differentiated according to size i.e. the smaller the fragment the farther it moves.

b)The DNA fragments thus separated according to size in agarose gel are taken out and stained with Ethidium Bromide which then on exposure to UV-Rays are visible as bright orange coloured bands. The separated band of DNA are then cut from agarose gel and extracted from gel piece. This isolation process is called as Elution.

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