Q. 44.1( 7 Votes )

A biotechno

Answer :

• The biotechnologist can ligate the foreign DNA at the Sal I of Tetracycline resistance gene in pBR322 vector.


• Since the Tetracycline resistance gene has 2 restriction sites, namely BamH I and Sal I, the foreign DNA can be ligated at any of these 2 sites.


Option B is incorrect as Pvu I is a restriction site present in the Ampicillin resistance gene.


Option C is incorrect as EcoRI is present within the promoter of Ampicillin resistance gene.


Option D is incorrect as Hind III is present within the promoter of Tetracycline.

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