Q. 44.1( 7 Votes )
• The biotechnologist can ligate the foreign DNA at the Sal I of Tetracycline resistance gene in pBR322 vector.
• Since the Tetracycline resistance gene has 2 restriction sites, namely BamH I and Sal I, the foreign DNA can be ligated at any of these 2 sites.
Option B is incorrect as Pvu I is a restriction site present in the Ampicillin resistance gene.
Option C is incorrect as EcoRI is present within the promoter of Ampicillin resistance gene.
Option D is incorrect as Hind III is present within the promoter of Tetracycline.
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