Q. 125.0( 1 Vote )

<span lang="EN-US

Answer :

a. Chromogenic substrate is used to identify recombinants and non-recombinants. The bacteria are grown in a medium with chromogenic substrate. Recombinants show no blue coloration while non-recombinants show blue coloured colonies.

b. This method is called “insertional inactivation” because the insertion of recombinant-DNA within the coding sequence β-galactosidase enzyme site results into inactivation of the enzyme.

Rate this question :

How useful is this solution?
We strive to provide quality solutions. Please rate us to serve you better.
Try our Mini CourseMaster Important Topics in 7 DaysLearn from IITians, NITians, Doctors & Academic Experts
Dedicated counsellor for each student
24X7 Doubt Resolution
Daily Report Card
Detailed Performance Evaluation
view all courses

Explain three steBiology - Board Papers

<span lang="EN-USBiology - Board Papers

<span lang="EN-USBiology - Board Papers