a. Chromogenic substrate is used to identify recombinants and non-recombinants. The bacteria are grown in a medium with chromogenic substrate. Recombinants show no blue coloration while non-recombinants show blue coloured colonies.
b. This method is called “insertional inactivation” because the insertion of recombinant-DNA within the coding sequence β-galactosidase enzyme site results into inactivation of the enzyme.
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